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Soothing/Anti-inflammatory Byungpul Extract, Byungpul Extract(PD) Centella Asiatica Extract 1-3% Product Name_ INCI Name_ Dosage_ Byungpul Extract™ Dermatologist’s Scoring On Day 0, patches containing 3% SLS solution were applied for 24 hours to induce erythema onto the forearm s of 8 female volunteers aged between 18 and 65. On Day 9, erythema was re-induced by applying 3% SLS solution. A cream containing 2% Byungpul Extract was applied to the irritated skin twice a day for 10 days. Erythema Value Scratch Assay in Human Fibroblast 60 50 40 30 20 10 0 % o f C o n t r o l Concentration (ppm) Control 20105 120 100 80 60 40 20 0 % o f C o n t r o l Concentration (ppm) Control 100502010 7 6 5 4 3 2 1 0 D e r m a t o l o g i s t ' s S c o r i n g SLS Day 1 Day 3 Day 5 Day 7 Day 9 SLS Day 10 E V a l u e 350 300 250 200 150 100 50 0 SLS Day 1 Day 3 Day 5 Day 7 Day 9 SLS Day 10 Time (Day) Time (Day) Wound Healing Effect – in vitro Evaluation Skin Soothing Effect – in vivo Evaluation Byungpul Extract Byungpul Extract Byungpul Extract Placebo Byungpul Extract Placebo Story Centella asiatica, commonly known as gotu kola, is a medicinal herb widely used Traditional Oriental Medicine as well as in Ayurvedic medicine for the treatment of skin diseases and for wound healing. According to folklore, injured tiger often rolls against C. asiatica for wound healing. Because of this, C. asiatica is well known for tiger’s herb in Asia. Scientific studies reported t hat topical application of C. asiatica on the wounded area increased the rate of healing by stimulating the production of type I colla gen. It is also reported that C. asiatica contains bioactive compounds which show anti-inflammatory activities. Properties of Byungpul Extract COX-2 Inhibition Activity in Macrophages (RAW 264.7) Anti-inflammatory Effect – in vitro Evaluation β-hexosaminidase Release Inhibition in Basophils (RBL-2H3) Anti-allergic Effect – in vitro Evaluation 2 4 h o c c l u s i v e S L S t r e a t m e n t 2 4 h o c c l u s i v e S L S t r e a t m e n t 2 4 h o c c l u s i v e S L S t r e a t m e n t 2 4 h o c c l u s i v e S L S t r e a t m e n t Day 0 Day 1 Day 3 Control Byungpul Extract 2% Byungpul Extract Anti-allergic W ound healing Anti-inflammatory 27