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Skin Brightening Scoti-Horsetail SPD Equisetum Fluviatile Extract 1-3% Product Name_ INCI Name_ Dosage_ Scoti-Horsetail™ Scoti- Horsetail Sk i n Br i g h t e n i n g A n t i - inflammatory A U 0.00 0.50 1.00 1.50 2.00 2.50 Minutes 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 O O O OH OH HO NO Synthesis Inhibition in Macrophages (RAW 264.7) Melanin Synthesis Inhibition in Melanocytes Inhibition of Tyrosinase Expression 120 100 80 60 40 20 0 % o f C o n t r o l Concentration (%, v/v) Control 210.5 % o f C o n t r o l Concentration (%, v/v) 120 100 80 60 40 20 0 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 L V a l u e D i ff e r e n c e Time(Day) Lightness Value ITA°(individual topology angle) δ(D56-D0)δ(D28-D0) Tyrosinase C 0.5 1 2 (%) β-actin 0.5 10.1Control 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0 I T A ° V a l u e Time(Day) δ(D56-D0)δ(D28-D0) Story Properties of Scoti-Horsetail Equisetum fluviatile, commonly known as water horsetail, is a perennial horsetail growing in a damp and swa mp area. It grows in great abundance in Great Britain and particularly in Scotland. They have hollow stems and lack proper leaves and flowers. The stems contain the constituents desired in skin care. E. fluviatile is one of the plants selected for the collaborative research project with Royal Botanic Garden Edinburgh and collected from Scotland. The extract from E. fluviatile shows anti-inflammatory and ski n brightening properties. A natural derivative of apigenin, protoapigenone was found in E. fluviatile as one of the active compounds. Active Compound of Scoti-Horsetail Molecular Structure of Protoapigenone Anti-inflammatory Effect – in vitro Evaluation 22 female volunteers aged between 30 and 55 years old applied a cream containing 2% Scoti-Horsetail twice a day on face for 56 days. Skin Brightening Effect – in vivo Evaluation on Facial Dark Spot Area Skin Brightening Effect – in vitro Evaluation Skin Brightening Effect – in vitro Evaluation Scoti-Horsetail Placebo Scoti-Horsetail Placebo * This is a product developed by the co-research project in associaton with the Royal Botanic Garden Edinburgh. Melanin Content Viability NO Viability Protoapigenone 25