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Skin Brightening BDS-Memil(PD) Polygonum Fagopyrum (Buckwheat) Sprout Extract 1-3% Product Name_ INCI Name_ Dosage_ BDS-Memil™ Polygonum fagopyrum, also called buckwheat, is a nutritious dietary source of protein with favorable amino a cid composition, vitamins, dietary fiber, and minerals. Buckwheat seeds and sprouts are known to contain high levels of anti-oxidants, in cluding rutin and anthocy- anin. BDS-Memil is an extract from buckwheat sprouts cultivated by Bio-Dynamic Sprout (BDS) System. BD S-Memil contains orientin, iso-orientin, rutin, and vitexin as active compounds. It shows anti-oxidant activity as well as melanin inhibition activity. Story DPPH Scavenging Activity Anti-oxidant Effect – in vitro Evaluation % o f C o n t r o l Concentration (%, v/v) 100 80 60 40 20 0 BDS-Memil 3 6 12 Melanin Synthesis Inhibition in Melanocytes Skin Brightening Effect – in vitro Evaluation NO Synthesis Inhibition in Macrophages (RAW264.7) Anti-inflammatory Effect – in vitro Evaluation % o f c o n t r o l 1 20.5 Control 1 20.5 Control Concentration (%, v/v) 120 100 80 60 40 20 0 Melanin Content % o f C o n t r o l Concentration (%, v/v) 120 100 80 60 40 20 0 Viability NO Cell Viability Skin Brightening Effect – in vivo Evaluation 21 female volunteers aged between 18 and 60 years old applied a cream containing 2% BDS-Memil twice a day on face. Melanin Index Lightness Value ITA°(individua l topology angle) M e l a n i n I n d e x Time(Day) 110 100 90 80 70 BDS-Memil Placebo D28 D56D0 L V a l u e D i ff e r e n c e 2.5 2 1.5 1 0.5 0 BDS-Memil Placebo δ(D56-D0)δ(D28-D0) I T A V a l u e ( % o f c o n t r o l ) Time(Day) 140 120 100 80 BDS-Memil Placebo D28 D56D0 BDS System (Bio-Dynamic Sprout System) Minutes A U 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 23.00 24.00 25.00 26.00 27.00 28.00 Iso-orientin Orientin Rutin Vitexin Active Compounds of BDS-Memil Time(Day) 22