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RiFerm® -HD Saccharomyces/Rice Ferment Filtrate 10-100% Product Name_ INCI Name_ Dosage_ RiFerm® Skin Hydration TEWL (Transepidermal water loss) Crow’s Feet Elasticity Nasolabial Fold Elasticity Before fermentation After fermentation Light microscope 400X (KRÜSS, Germany) 0.1% 0.5% 1%Control Rice water, leftover milky white water after washing rice, has been traditionally used as facial and hair w ash for centuries by Asian women to improve skin complexion and hair condition. Nutritious rice water fermented by Saccharomyces bec ame a favorable ingredient for cosmetics through chemical and physical changes during the process. RiFerm ® exhibits strong anti-oxidant and anti-wrinkle activities in vitro and shows significant skin hydration and skin elasticity in vivo. Story Rice starch degradation during fermentation ROS Generation Inhibition Activity Anti-oxidant Effect – in vitro Evaluation Collagen Synthesis Activity of Before and After Fermentation Anti-wrinkle Effect – in vitro Evaluation After Fermentation Before Fermentation P I P ( % o f c o n t r o l ) 0.5 10.1Control Concentration (%, v/v) 160 140 120 100 80 60 40 20 0 0 10 20 30 40 50 60 70 80 90 1200 1000 800 600 400 200 0 D C F fl u c o r s e c e n c e i n t e n s i t y Time(min) Skin Moisturizing Effect – in vivo Evaluation 30 volunteers aged between 35 to 60 years old applied a product containing 94% RiFerm twice a day for 56 days on face. S k i n h y d r a t i o n ( a . u . ) Time(Day) 45 40 35 30 25 20 15 10 5 0 Placebo RiFerm® Placebo RiFerm® 30 volunteers aged between 35 to 60 years old applied a product containing 94% RiFerm twice a day for 56 days . Skin Firming Effect – in vivo Evaluation D0 D14 *** *** D28 *** *** D56 *** *** *** S k i n E l a s t i c i t y Time(Day) 0.70 0.69 0.68 0.67 0.66 0.65 0.64 0.63 0.62 0.61 Placebo RiFerm® D14 D28 D56D0 *** *** *** *** *** *** S k i n T E W L ( g / m 2 h ) Time(Day) 18 16 14 12 10 8 6 4 2 0 D0 D28 *** ** D14 ** D56 *** * *** P value < 0.05*, < 0.01 **, < 0.001**P value < 0.05*, < 0.01 **, < 0.001*** P value< 0.05*, < 0.01 **, < 0.001*** S k i n E l a s t i c i t y Time(Day) 0.71 0.70 0.69 0.68 0.67 0.66 0.65 0.64 Placebo RiFerm® D14 D28 D56D0 *** *** *** *** *** *** * P value< 0.05*, < 0.01 **, < 0.001*** Anti-aging 19