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Phytoceutical(PD) Lycium Chinense Stem Extract 1-3% Product Name_ INCI Name_ Dosage_ Lycium chinense is mainly distributed throughout East Asia and in particular throughout southern China, Kor ea, and Japan. The bright orange-red ellipsoid berries are one of the most popular tonic herbs and have been frequently used in orie ntal herbal medicine for thousands of years. L. chinensis is rich in vitamins B and C, flavonoids, carotenes and many other bioactive com pounds. Phytoceutical is an extract from the stem of L. chinense and shows anti-oxidant, anti-inflammatory and anti-wrinkle activitie s. N-feruloyltyramine was identified from L. chinense as one of the active compounds with anti-wrinkle activity. Story Phytoceutical™ A U 0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 0.045 0.050 Minutes 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 Identification of Active Compound Molecular Structure of N-feruloyltyramine HO OH H 3 C O O N H Anti-oxidant Effect – in vitro Evaluation Anti-wrinkle Effect – in vitro Evaluatio n Anti-inflammatory Effect – in vitro Evaluation ROS Generation Inhibition Activity Collagen Synthesis Activity in Fibroblasts NO Synthesis Inhibition in Macrophages (RAW264.7) 600 500 400 300 200 100 0 D C F F l u o r e s c e n c e I n t e n s i t y 5040302010 60 70 80 90 100 Time (Min) % o f C o n t r o l Concentration (ppm) Concentration (ppm) 140 120 100 80 60 40 20 0 PIP 10 50 100Control 10 ppm 50 ppm 100 ppmControl Viability % o f C o n t r o l 140 120 100 80 60 40 20 0 NO 10 50 100Control Viability Anti-wrinkle Effect – in vivo Evaluation 22 female volunteers aged between 35 and 55 years old applied a cream containing 2% Phytoceutical twice a day fo r 56 days to the eye contour area. Skin Elasticity Wrinkle Depth S k i n E l a s t i c i t y ( % o f C o n t r o l ) D56D28D0 Time (Day) 110 105 100 95 90 Phytoceutical PlaceboPhytoceutical Placebo W r i n k l e D e p t h ( % o f C o n t r o l ) D56D28D0 Time (Day) 102 100 98 96 94 92 90 Anti-aging N-feruloyltyramine 17