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Beauty Plus One-F™ Product Name_ INCI Name_ Dosage_ Anti-wrinkle Effect – in vivo Evaluation 21 female volunteers aged between 45 and 65 years old applied a cream containing 2% Beauty Plus One-F twic e a day on a face. Beauty Plus One-F, Beauty Plus One-F(PD) Lactobacillus/Punica Granatum Fruit Ferment Extract 1-3% Punica granatum, also known as pomegranate, is native to Persia and is widely cultivated in the Middle East an d Asian countries including Iran, Iraq, India, China, Japan, and Korea. The sweet and sour seeds surrounded by a fleshy aril are con sumed fresh or as a juice. Pomegranate is a good source of vitamins and polyphenolic compounds such as ellagitannins and flavonoid s, which are very powerful antioxidants. Beauty Plus One-F is a natural ingredient from fermented pomegranate fruit processed by Korea n Traditional Solid Fermen- tation. Beauty Plus One-F shows anti-oxidant activity as well as anti-wrinkle activity. Story Properties of Beauty Plus One-F The penetration of the active compounds depends on the molecular weight. It is difficult for most polymers to penetrate the epidermis because of big molecular weight. Beauty Plus One-F were processed by Korean Traditional Solid Fermentation method in order to convert large biopolymers in the raw material to small compounds. Fermentation Process Epidermis Dermis Hypodermis Epidermis Dermis Hypodermis Naturally fermentated Bio Polymer Small molecules Big molecules Beauty Plus One-F F ermen t a ton An t-o xidan t polyphenols An t-wrink le Anti-oxidant Effect – in vitro Evaluation Anti-wrinkle Effect – in vitro Evaluation Anti-wrinkle Effect – in vitro Evaluation DPPH Scavenging Activity % o f C o n t r o l Concentration (%, v/v) 100 90 80 70 60 50 40 30 20 10 0 Beauty Plus One-F 0.04 0.08 0.17 0.33 0.670.02 Dermatologist’s Score Photograph Visualization D e r m a t o l o g i s t ' s S c o r e Time (Day) 4.5 4.4 4.3 4.2 4.1 4 3.9 3.8 3.7 3.6 3.5 Beauty Plus One-F Placebo D0 D28 D56 Collagenase Inhibition Activity % o f C o n t r o l Concentration (%, v/v) 70 60 50 40 30 20 10 0 Beauty Plus One-F 0.01 0.025 0.050.005 Collagen Synthesis Activity in Fibroblast Beauty Plus One-F 2% Before After 10 50 100Control P I P ( % o f C o n t r o l ) Concentration (ppm) 140 120 100 80 60 40 20 0 Beauty Plus One-F Anti-aging 13