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Leafresh™ Leafresh(NB) Nelumbo Nucifera Leaf Extract 1-3% Product Name_ INCI Name_ Dosage_ Anti-aging Nelumbo nucifera, also known as a lotus, is one of aquatic plant which rises through muddy water and produce pure and uncontaminated flowers. This feature makes it a symbol of pure and resurection. Lotus leaf is widely consumed as a food, tea and folk medicine. It is believed that all diseases are recovered by drinking the tea consistently for a long time. Leafresh protect s skin from environmental factors such UV radiation and oxidative stresses. Story Properties of Leafresh HPLC chromatogram of LeafreshPhotoaging Mechanism Leafresh An t-polluton An t-o xidan t Skin Brigh t ening An t-phot oaging Minutes A U 0.00 0.50 1.00 1.50 2.00 2.50 3.00 23.0021.00 22.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 ROS Generation Inhibition Activity Anti-photoaging Effect – in vitro Evaluation Hydroxyl Radical Scavenging Activity Anti-oxidant Effect – in vitro Evaluation 3.13 6.25 12.501.56 % o f c o n t r o l Concentration (%, v/v) 30.0 25.0 20.0 15.0 10.0 5.0 0 Leafresh Anti-oxidant Effect – in vitro Evaluation Anti-oxidant Effect – in vitro Evaluation DPPH Scavenging Activity % o f c o n t r o l Concentration (%, v/v) 100 90 80 70 60 50 40 30 20 10 0 Leafresh 1.25 2.50 5.000.63 % o f c o n t r o l Concentration (%, v/v) 100 90 80 70 60 50 40 30 20 10 0 Leafresh 1.56 3.13 6.25 12.50 Superoxide Scavenging Activity Skin Brightening Activity – in vitro Evaluation Melanin Synthesis Inhibition in Melanocytes 0.5 1 2Control % o f c o n t r o l Concentration (%, v/v) 140 120 100 80 60 40 20 0 Melanin Content Cell Viability * mJ/cm2 : Radiant Energy Density (millijoules per square centimeter) D C F F l u o r e s c e n c e I n t e n s i t y ( % ) 1 20.5 UVB- Control UVB 100mJ/cm 2 Concentration (%, v/v) 120 100 80 60 40 20 0 * UV radiation causes skin aging Free radicals↑ Photodamage (Inflammation, wrinkle, and pigmentation) UVB UVA Quercetin 3-O-lathyroside Reynoutrin Hyperoside Isoquercitrin Epidermis Dermis Epidermis Dermis 11